Effect of the biofertilizer Humina on yield components of cultivar IACuba-41 (Oryza sativa L.) in a Vertisol in Río Cauto, Granma
DOI:
https://doi.org/10.56124/Keywords:
Biofertilizer, crop, production, yield, sustainabilityAbstract
The use of agricultural products has increased significantly due to the benefits they provide, such as higher yields and sustainable environmental management of the agroecosystem. The case study was conducted at the Enrique Moreno Credit and Services Cooperative, located in the Las Galaxias area of Río Cauto, Granma, Cuba, during the period from January to April 2023. The objective of this study was to evaluate the IACuba-41 cultivar under two treatments: foliar application of Humina (4 L ha⁻¹) and a complete control. The bioproduct in question was applied four times, starting 15 days after germination and continuing until 10 days before flowering. In this study, we quantified the number of empty and filled grains per panicle and determined panicle density, 1,000-grain weight, and yield (t ha⁻¹). The statistical analysis included Student’s t-test for comparing means and multiple linear regression to determine the relationships between the predictor variables and yield. The application of Humina resulted in a notable increase in all evaluated variables compared to the control group. However, multiple linear regression did not detect statistical significance in the linear relationship between components and yield for each treatment separately. Full grains per panicle had the greatest influence on crop yield. The bioproduct in question demonstrated remarkable efficacy in optimizing yield, as evidenced by the results obtained at IACuba-41. The implementation of this system, in combination with the provision of specialized technical advice, could constitute a viable and sustainable alternative for low-input rice production in the rice-growing regions of eastern Cuba.
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References
Ahmad, F., Ahmad, I., & Khan, M. S. (2008). Screening of free-living rhizospheric bacteria for their multiple plant growth promoting activities. Microbiological Research, 163(2), 173–181. https://doi.org/10.1016/j.micres.2006.04.001
Altomare, C., Norvell, W. A., Björkman, T., & Harman, G. E. (1999). Solubilization of phosphates and micronutrients by the plant-growth-promoting and biocontrol fungus Trichoderma harzianum Rifai 1295-22. Applied and Environmental Microbiology, 65(7), 2926–2933. https://doi.org/10.1128/AEM.65.7.2926-2933.1999
Austin, P. C., Eekhout, I., & van Buuren, S. (2025). Evaluating the median p-value method for assessing statistical significance in randomized controlled trials. Journal of Statistical Computation and Simulation, 95(3), 523–541. https://doi.org/10.1080/02664763.2024.2418473
Beneduzi, A., Ambrosini, A., & Passaglia, L. M. P. (2012). Plant growth-promoting rhizobacteria (PGPR): Their potential as antagonists and biocontrol agents. Genetics and Molecular Biology, 35(4 Suppl), 1044–1051. https://doi.org/10.1590/S1415-47572012000600020
Benítez, T., Rincón, A. M., Limón, M. C., & Codón, A. C. (2004). Biocontrol mechanisms of Trichoderma strains. International Microbiology, 7(4), 249–260. PMID: 15666245. https://pubmed.ncbi.nlm.nih.gov/15666245/
Bera, B., Bokado, K., Barkha, Bag, A. G., & Naga, T. T. R. (2024). Effect of farmyard manure and humic acid on growth and yield of rice (Oryza sativa L.). Plant Science Today, 11(4), 1596–1604. https://doi.org/10.14719/pst.3755
Caipo, Y., Gutiérrez, A., & Julca, A. (2015). Optimización por diseño de mezclas de la aceptabilidad de una barra energética a base de quinua (Chenopodium quinoa), kiwicha (Amaranthus caudatus) y cañihua (Chenopodium pallidicaule) evaluada en niños. Agroindustrial Science, 5(1), 61–67. https://doi.org/10.17268/agroind.science.2015.01.06
Chen, H., Ruan, Y., & Jia, Z. (2025). A Meta-Analysis of 30 Years in China and Micro-District Experiments Shows Organic Fertilizer Quantification Combined with Chemical Fertilizer Reduction Enhances Rice Yield on Saline-Alkali Land. Rice Science, 32(2), 259–272. https://doi.org/10.1016/j.rsci.2025.01.004
Contreras-Cornejo, H. A., Macías-Rodríguez, L., Cortés-Penagos, C., & López-Bucio, J. (2009). Trichoderma virens, a plant beneficial fungus, enhances biomass production and promotes lateral root growth through an auxin-dependent mechanism in Arabidopsis. Plant Physiology, 149(3), 1579–1592. https://doi.org/10.1104/pp.108.130369
De Moura, O. V. T., Berbara, R. L. L., De Oliveira Torchia, D. F., Da Silva, H. F. O., Van Tol De Castro, T. A., Tavares, O. C. H., Rodrigues, N. F., Zonta, E., Santos, L. A., & García, A. C. (2023). Humic foliar application as sustainable technology for improving the growth, yield, and abiotic stress protection of agricultural crops: A review. Journal of the Saudi Society of Agricultural Sciences, 22(8), 493–513. https://doi.org/10.1016/j.jssas.2023.05.001
Di Rienzo, J. A., Casanoves, F., Balzarini, M. G., González, L., Tablada, M., & Robledo, C. W. (2020). InfoStat (Versión 2020) [Software]. Centro de Transferencia InfoStat, FCA, Universidad Nacional de Córdoba. http://www.infostat.com.ar
Efthimiadou, A., Katsenios, N., Chanioti, S., Karatassiou, M., Kougias, T. G., Bartzialis, C., Tsivelika, N., Tzerakis, C., Xatzitheodorou, A., Tsakalidou, E., Ntatsi, G., & Savvas, D. (2020). Effect of foliar and soil application of plant growth promoting bacteria (Azotobacter chroococcum, Bacillus subtilis and Bacillus megatherium) on growth, physiology, yield and seed quality of maize under Mediterranean conditions. Scientific Reports, 10, 21060. https://doi.org/10.1038/s41598-020-78034-6
El-Aty, M. S. A., Abo-Youssef, M. I., Sorour, F. A., Salem, M., Gomma, M. A., Ibrahim, O. M., Yaghoubi Khanghahi, M., Al-Qahtani, W. H., Abdel-Maksoud, M. A., & El-Tahan, A. M. (2024). Performance and stability for grain yield and its components of some rice cultivars under various environments. Agronomy, 14(9), 2137. https://doi.org/10.3390/agronomy14092137
Frontela-Zambrana, J. M., Maqueira López, L., Pérez León, N. de J., & Páez López, A. (2025). Productive Rice cultivar IACuba 41, in state seed production area in Los Palacios. Avances, 27(2), 166-178. https://avances.pinar.cu/index.php/publicaciones/article/view/911/2197
Fukagawa, N. K., & Ziska, L. H. (2019). Rice: Importance for Global Nutrition. Journal of Nutritional Science and Vitaminology, 65(Supplement), S2–S3. https://doi.org/10.3177/jnsv.65.S2
Glick, B. R. (2012). Plant growth-promoting bacteria: Mechanisms and applications. Scientifica, 2012, 963401. https://doi.org/10.6064/2012/963401
Gopalakrishnan, S., Srinivas, V., Alekhya, G., & Prakash, B. (2015). Effect of plant growth-promoting Streptomyces sp. on growth promotion and grain yield in chickpea (Cicer arietinum L.). 3 Biotech, 5(6), 799–806. https://doi.org/10.1007/s13205-015-0283-8
Harman, G. E., Howell, C. R., Viterbo, A., Chet, I., & Lorito, M. (2004). Trichoderma species — opportunistic, avirulent plant symbionts. Nature Reviews Microbiology, 2(1), 43–56. https://doi.org/10.1038/nrmicro797
Hashim, N., Mohd Ali, M., Mahadi, M. R., Abdullah, A. F., Wayayok, A., Mohd Kassim, M. S., & Jamaluddin, A. (2024). Smart farming for sustainable rice production: An insight into application, challenge, and future prospect. Rice Science, 31(1), 47–61. https://doi.org/10.1016/j.rsci.2023.08.004
Hernández, R., Robles, C., Calderín, A., Guridi, F., Reynaldo, I. M., & González, D. (2018). Efectos antiestrés de ácidos húmicos de vermicompost en dos cultivares de arroz (Oryza sativa L.). Cultivos Tropicales, 39(2), 65–74. http://scielo.sld.cu/scielo.php?script=sci_arttext&pid=S0258-59362018000200009
Hernández-Jiménez, A., Pérez-Jiménez, J. M., Bosch-Infante, D., & Castro Speck, N. (2019). La clasificación de suelos de Cuba: énfasis en la versión de 2015. Cultivos Tropicales, 40(1), a15-e15. Recuperado a partir de https://ediciones.inca.edu.cu/index.php/ediciones/article/view/1504
Howell, C. R. (2003). Mechanisms employed by Trichoderma species in the biological control of plant diseases: The history and evolution of current concepts. Plant Disease, 87(1), 4–10. https://doi.org/10.1094/PDIS.2003.87.1.4
IIG-CUBA: Instructivo Técnico del Cultivo del Arroz (2020). Instituto de Investigaciones de Granos (IIG), Imprenta EAS-MINAG, La Habana, Cuba, 142 p.
Izquierdo, J., Arriagada, O., Garcia-Pintos, G., Ortiz, R., Garcia-Pintos, M., Buschiazzo, D., Mendez-Gonzalez, L., Rizzo, J., Pancotto, V., Bouza, C., Godoy, T., Celaya, P., Jaurena, G., & Garcia-Mina, J.-M. (2024). On-farm foliar application of a humic biostimulant increases the yield of rice. Agronomy Journal, 116(5), 2551–2563. https://doi.org/10.1002/agj2.21641
Joneer, A., & Sam, L. M. (2022). Effect of humic acid on growth and yield of Tadong upland rice. Science, Engineering and Health Studies, 16, 22030011. https://doi.org/10.14456/sehs.2022.50
Kavitha, M. P., & Janaki, D. (2018). Crop – weed modeling and fitting multiple regression equation in system of rice intensification. International Journal of Current Microbiology and Applied Sciences, 7(9), 3005–3013. https://doi.org/10.20546/ijcmas.2018.709.374
Lakra, N. U., Samadhiya, V. K., Parghanha, S., & Soni, P. (2025). Influence of foliar-applied liquified humic acid on growth, yield, and yield components of rice in Vertisol conditions of Chhattisgarh. International Journal of Research in Agronomy, 8(11), 791–796. https://doi.org/10.33545/2618060X.2025.v8.i11k.4279
Ma, Y., Cheng, X., & Zhang, Y. (2024). The impact of humic acid fertilizers on crop yield and nitrogen use efficiency: A meta-analysis. Agronomy, 14(12), 2763. https://doi.org/10.3390/agronomy14122763
Mastouri, F., Björkman, T., & Harman, G. E. (2010). Seed treatment with Trichoderma harzianum alleviates biotic, abiotic, and physiological stresses in germinating seeds and seedlings. Phytopathology, 100(11), 1213–1221. https://doi.org/10.1094/PHYTO-03-10-0091
Muthayya, S., Sugimoto, J. D., Montgomery, S., & Maberly, G. F. (2014). An overview of global rice production, supply, trade, and consumption. Annals of the New York Academy of Sciences, 1324(1), 7–14. https://doi.org/10.1111/nyas.12540
Oh, S., & Cho, Y.-S. (2025). Effects of water management practices on rice grain quality and pest-disease incidence in environmentally friendly cultivation systems. Agriculture, 15(21), 2244. https://doi.org/10.3390/agriculture15212244
Puteh, A. B., Mondal, M. M. A., Ismail, M. R., & Latif, M. A. (2014). Grain sterility in relation to dry mass production and distribution in rice (Oryza sativa L.). BioMed Research International, 2014, Article 302179. https://doi.org/10.1155/2014/302179
Revillas, J. J., Rodelas, B., Pozo, C., Martínez-Toledo, M. V., & González-López, J. (2000). Production of B-group vitamins by two Azotobacter strains with phenolic compounds as sole carbon source under diazotrophic and nondiazotrophic conditions. Journal of Applied Microbiology, 89(3), 486–493. https://doi.org/10.1046/j.1365-2672.2000.01139.x
Richardson, A. E., Barea, J. M., McNeill, A. M., & Prigent-Combaret, C. (2009). Acquisition of phosphorus and nitrogen in the rhizosphere and plant growth promotion by microorganisms. Plant and Soil, 321(1-4), 305–339. https://doi.org/10.1007/s11104-009-9895-2
Sima, N. Q., Harmel, R. D., Fang, Q. X., Ma, L., & Andales, A. A. (2018). A modified F-test for evaluating model performance by including both experimental and simulation uncertainties. Environmental Modelling & Software, 104, 236–248. https://doi.org/10.1016/j.envsoft.2018.03.011
Saha, B. K., Islam, Z., & Basak, R. K. (2013). Growth and yield of rice (Oryza sativa) as influenced by humic acid and poultry manure. International Journal of Current Microbiology and Applied Sciences, 2(12), 241–248. https://doi.org/10.13189/ujps.2013.010304
Singh, D., Suhag, M., & Dhaka, A. K. (2025). Insights of agricultural sustainability by the use of earthworms. Discover Applied Sciences, 7(1), 322. https://doi.org/10.1007/s44279-025-00322-4
Stewart, A., & Hill, R. (2014). Applications of Trichoderma in plant growth promotion. En V. K. Gupta, M. Schmoll, A. Herrera-Estrella, R. S. Upadhyay, I. Druzhinina, & M. G. Tuohy (Eds.), Biotechnology and Biology of Trichoderma (pp. 415–428). Elsevier. https://doi.org/10.1016/B978-0-444-59576-8.00031-X
Vejan, P., Abdullah, R., Khadiran, T., Ismail, S., & Nasrulhaq Boyce, A. (2016). Role of plant growth promoting rhizobacteria in agricultural sustainability—A review. Molecules, 21(5), 573. https://doi.org/10.3390/molecules21050573
Zheng, E., Qin, M., Zhang, Z., & Xu, T. (2022). Humic Acid Fertilizer Incorporation Increases Rice Radiation Use, Growth, and Yield: A Case Study on the Songnen Plain, China. Agriculture, 12(5), 653. https://doi.org/10.3390/agriculture12050653
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Copyright (c) 2026 Lisandra Cuervo Bárzaga, Sergio Rodríguez Rodríguez, Wilson Geobel Ceiro Catasú, María de los Ángeles Jiménez Pizarro, Sahily Cano Llorente, Gerardo Cueto Aldana, Arisdalia Alarcón Alba
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